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Fall Chem501 Seminar

UT Host:  Dr. Bhavya Sharma

Speaker:  Dr. Dan Fu

Assistant Professor of Chemistry

University of Washington

Title:  “Quantitative chemical imaging of structure and function in living biological samples –    from single cells to animals”

Abstract:  Biological systems are highly complex. The complexity arises not only from intricate spatial organizations of different types of cells, but also from the heterogeneous nature of living cells. It is increasingly recognized that cell heterogeneity plays an important role in many pathophysiological processes. However, phenotypic variations of individual cells are often intractable by traditional analytical techniques, despite their exquisite sensitivity and specificity. The main obstacles are the limited amount of analyte retrievable from a single cell and the need for noninvasive in situ analysis in order to preserve cell function. Additionally, cell phenotype and function are strongly dependent on their microenvironment. The ideal analytical technique should be able to noninvasively probe chemical concentrations in cells at subcellular resolution within intact tissue or animal. Towards that goal, we focus on the development of label-free optical imaging techniques that enable us to perform quantitative chemical and functional measurements at high spatial and temporal resolution from cultured cells to living animals. We applied broadband and high sensitivity stimulated Raman scattering microscopy, an emerging chemical imaging tool, to study cell growth, cell metabolism, as well as disease processes. We are also developing transient absorption microscopy to study a wide range of red blood cell associated functional processes such as neurovascular coupling in mouse brain. Together, we aim to build an integrated chemical imaging platform that is coupled with advanced data analysis including machine learning for comprehensive structural and functional imaging of living biological samples with single cell resolution.

Bio:  Dr. Dan Fu is Assistant professor of Chemistry at the University of Washington. He received his bachelor's degree from Peking University in China. In 2009, he completed his Ph.D study at Princeton University under the supervision of Professor Warren Warren, working on the development of label-free multiphoton absorption microscopy methods. After that, he worked as a postdoctoral associate at the G.R.Harrison Spectroscopy Lab led by the late professor Michael Feld at Massachusssets Institute of Technology, where he investigated quantitative phase microscopy and its applications to live cell imaging. In 2010, Dr. Fu moved to Harvard University to work with Professor Sunney Xie as a postdoctoral fellow, where he developed multiplex stimulated Raman scattering microscopy and hyperspectral stimulated Raman scattering microscopy. Dr. Fu joined the faculty of the University of Washington in the summer of 2015. Currently his main research interests are the development and applications of quantitative chemical imaging tools to various pathophysiological processes of living biological specimens at single cell resolution. He is a recipient of the Beckman Young Investigator Award, the NSF Career Award, and the NIH MIRA Award.

Thursday, November 14 at 3:45pm to 5:00pm

Buehler Hall, 555
1420 Circle Drive, Knoxville, TN 37996

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Linda Sherman

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