Fall Chem501 Seminar
UT Host: Dr. Joshua Baccile
Speaker: Dr. Darci Trader
Assistant Professor/Medicinal Chemistry and Molecular Pharmacology
Title: “Methods to Evaluate and Perturb the Activity of the Human Proteasome with Small Molecules”
Abstract: The degradation of proteins is an essential cellular process. The proteasome, a multi-catalytic enzyme, is mainly responsible for degrading proteins that are no longer required by the cell. My lab has focused on the development of activity probes that can monitor real-time proteasome activity biochemically and in live cells. These probes can be applied in variety of analytical methods including confocal microscopy, flow cytometry, and fluorescent plate readers. With these probes, we have discovered a variety of proteasome stimulators and binders to non-catalytic subunits. The recently discovered proteasome stimulators have been applied to determine the effects of increasing the ubiquitin-independent degradation of proteins. Moving forward, we are now analyzing how these small molecule stimulators can affect the accumulation of unwanted proteins, including alpha-synuclein.
Bio: -B.S. from Southern Illinois University-Edwardsville, Edwardsville, IL in 2007
-Ph.D. from Indiana University, Advisor: Erin E. Carlson in 2013
-Postoc from The Scripps Research Institute, Advisor: Thomas Kodadek finished in 2016.
-Current position: Assistant Professor of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN
-The Trader lab is focused on discovering new tools for monitor proteasome activity and understand how its activity can perturb for a desired therapeutic response.
-We have published a number of exciting manuscripts including the development of a immunoproteasome activity probe that can be used in live-cells (J. Am. Chem. Soc. 2019, 141 (13), 5252-5260.) and studies to understand how stimulation of the endogenous activity of the proteasome can have a positive effect on protein accumulation diseases (RSC Chem. Biol. 2021, 2, 636-644.).
Thursday, October 14, 2021 at 4:30pm to 5:45pm
Buehler Hall, 555
1420 Circle Drive, Knoxville, TN 37996